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Image Search Results
Journal: Frontiers in Immunology
Article Title: Response to Oxidative Burst-Induced Hypoxia Is Associated With Macrophage Inflammatory Profiles as Revealed by Cellular Genome-Wide Association
doi: 10.3389/fimmu.2021.688503
Figure Lengend Snippet: Phenotypic characteristics of harvested cells after six days of in vitro incubation in serum-free media supplemented with recombinant bovine granulocyte-macrophage colony-stimulating factor. (A) Autofluorescence of Monocyte-Derived Macrophages (MDMs) (dark grey) versus monocyte (light grey), exited by blue laser and the emission was measured by 533/30 filter. (B) Expression of CD205 on MDMs. The harvested cells were stained with phytoerythrin (PE) conjugated anti-bovine CD-205 (dark grey) and compared against the unstained sample (light grey). (C) Expression of CD14 on MDMs. The harvested cells were stained with Alexa Flour 647 conjugated anti-human CD-14 (Clone TÜK4) (dark grey) versus the unstained sample (light grey). The cells were analyzed in BD Accuri™ C6 cytometer. The data from the flow cytometer were analyzed and graphed using FlowJo (v. 10).
Article Snippet: The labelled samples were analyzed using the BD Accuri C6 flow cytometer (Becton Dickinson, Franklin Lakes, NJ) and the data from the flow cytometer were analyzed by
Techniques: In Vitro, Incubation, Recombinant, Derivative Assay, Expressing, Staining, Cytometry, Flow Cytometry